Journal: BMC Molecular and Cell Biology

Article Title: PGRMC1 phosphorylation affects cell shape, motility, glycolysis, mitochondrial form and function, and tumor growth

doi: 10.1186/s12860-020-00256-3

Figure Lengend Snippet: RPPA measurements of protein and phosphorylated protein levels. a - d , f - h Average RPPA normalized fluorescent intensity (NFI) from the indicated antibodies (described in methods) is plotted from 6 replicate measurements. NFI is normalized to protein content. Statistical calculations for normally distributed data were made using one way ANOVA, and post-hoc Bonferroni (BF) for equal variances (all variances were equal). For non-parametric data, Kruskall-Wallis (KW) pairwise comparisons were calculated for 24 unrelated samples. * p < 0.05; ** p < 0.01, *** p < 0.001. Non-phosphorylated BAD levels could not be accurately determined because signal values were less than three times background. e The ratio of average NFI of D relative to C. Labels follow the above

Article Snippet: The following primary antibodies (provider and reagent number, dilution) were used: BAD (CST 9239, 1:200), BAD-P-Ser112 (CST 5284, 1:100), BAD-P-Ser136 (CST 4366, 1:100), FAK1-P-Tyr577 (Invitrogen 44-614ZG, 1:100), FAK1-P-Tyr861 (Epitomics 2153–1, 1:100), GSK3beta (CST 9315, 1:200), GSK3beta-P-Ser9 (CST 9336, 1:100), HSF1 (Epitomics 2043–1, 1:1000).

Techniques: